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Withdraw the syringe plunger to ensure that you are not in the bladder or intestines. If nothing is withdrawn inject the material slowly. If you accidentally enter the bladder or intestines withdraw and discard the needle and syringe.

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Intravenous injections Intravenous injections are usually made photso the dorsal tail vein. Warm the tail by immersing it in warm water or placing the animal under a heat lamp. The tail vein is easier to see in non-pigmented mice.

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A fine gauge needle should be used for this procedure. Signs of pain in the mouse.

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Pain relief For a detailed discussion of pain relief in mice refer to module 2. Drugs can be administered in water, in jello, as oral drops, by gavage or injected.

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Drugs administered in water may be broken down in water, or insufficient quantities may be taken due to poor solubility in water or palatability problems. Anesthesia In general inhalant anesthetics are safer than injectable anesthetics.

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Halothane and isoflurane are the safest ones to use. Methoxyflurane is no longer available.

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Use of ether at Johns Hopkins University is subject to restrictions due to safety concerns. Ketamine and xylazine is a common injectable anesthetic combination.

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Sodium pentobarbital can be used, but it has a narrow safety margin and is associated with a prolonged recovery period. For details on anesthetic techniques refer to the rodent surgery module.

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Euthanasia Euthanasia in mice is most often performed by carbon dioxide asphyxiation or overdose of an anesthetic agent. Use of cervical dislocation or decapitation in absence of deep anesthesia must be scientifically justified. All individuals performing euthanasia must be properly trained.

Individuals must also ensure that animals are dead before the carcass is anv. Exsanguination or opening the thoracic cavity will ensure death.

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AVMA Panel on euthanasia report. Diseases of mice are usually handled as a herd colony health problem rather than on an individual animal basis.

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The goal is to prevent introduction of a disease into a colony cute black teen nudes than to treat animals after disease outbreak. Disease prevention is practiced by institution of a disease surveillance sentinel program based on serological and microscopic diagnosis of very big and fat vagina photos in a representative sample of animals.

Due to the widespread movement of animals all over the world with advent of genetic manipulation of animals, the possibility of introducing disease agents in a colony has markedly increased. The expanded use of genetically modified and immunocompromised animals greatly exacerbates the problem.

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Furthermore the practice of transplanting tumor material into mice provides a portal where these agents can be introduced into animal, especially if the tumors are not screened for adventitious infectious agents. Some important mouse diseases are discussed very big and fat vagina photos to draw attention to the need to adhere to practices recommended download video xxxx naket the veterinary staff to avoid these diseases.

Pinworms Pinworms Syphacia and Aspicularis inhabit the intestine cecum, rectum, colon and have a direct lifecycle.

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The eggs are particularly resistant and survive for a long time in the environment. The disease is usually subclinical being marked in weanlings and immunocompromised animals.

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Symptoms include poor body condition, rough hair coat, reduced growth rate and rectal prolapse. Infection with pinworms has a negative impact on gastrointestinal, growth, behavioral and immunology studies.

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Affected animals are scruffy, pruritic itchyloose hair and have scratch wounds, which can become infected with bacteria. There are changes in the immune responses of affected animals. Mouse hepatitis virus This is a viral disease of mice that affects multiple organs.

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